Wednesday, February 3, 2010

What is the difference between an RNA transcript and mRNA?

Supposedly eukaryotes have both, while prokaryotes go straight from RNA transcript to mRNA. Any ideas?What is the difference between an RNA transcript and mRNA?
RNA transcript (pre-mRNA) is a single stranded copy of antisense strand of DNA (with U instead of T). In eukaryote transcript RNA goes through a splicing, when introns are excised from the molecule and all the exons are put together and also 3'poly(A) tail and 5'-cap are added.


Almost forgot... There are also 3' and 5' UTR (untranslated regions) that come before and after the coding sequence. What is the difference between an RNA transcript and mRNA?
mRNAs (messenger RNAs) are a kind of RNA transcript. Examples of other kinds are ribosomal RNAs (which make up the catalytic parts of the ribosomes) and transfer RNAs (which link codons to corresponding amino acids). All of these types of RNAs are transcripts produced from a DNA template. Prokayotes and eukaryotes both have all three types of RNAs. I'm not sure where you heard this other definition from but I don't think it's right...
RNA transcript is the copy of RNA from DNA (occurs in the nucleus in eukaryotes). RNA transcript goes through modifications within the nucleus before it is released into the cell as mRNA. Since there is no nucleus in prokaryotes, they just use RNA transcript.
I think mRNA is messenger RNA and is a copy of RNA that transfers info. I guess prokaryotes don't need it?

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